Trustworthy and antivirus don't belong in the same sentence. The point of these mass online scanning sites is to detect viruses and share them with antivirus companies. Also most AVs will throw out false positives on anything packed with UPX.
Back in the XP days when I would make WPI's I would UPX silent installers to fit on a 700mb disk and was a complete disaster once any AV was installed.
What I suggest is a Slim VM environment with several of the leading AVs on them
Most people use the following:
-bit defender
-Norton
-McAfee
-avg
-avast
-avira
-kasperky
-malwarebytes
However most systems don't do well with several AVs on one machine so multiple VMs, update and then take offline, use a machine freeze app like deepfreeze so when you shutdown the VM all changes are removed. Test and run your virii to ensure the file is undetected as a file and at runtime. When you reboot your VM there is no trace and no internet for the AVs to send it out for further exploration
If you want to share your file with AVs you have a few that I can think of off the top of my head, dunno if they still exist haven't used them in years...
Virustotal, novirusthanks, virusscan jotti, kasperky online, Norton online, bitdefender online, and I think avira has an online scanner as well